ABSTRACT
A total of 200 breast tissues which included 5 normals, 55 benign and 140 malignant lesions were stained for Argyrophilic Nucleolar Organiser Regions (AGNORs). A comparison of the AGNOR values with histologic variables, viz., tumor type, size nuclear grade, desmoplasia, elastosis, lymph node metastasis and Oestrogen and Progesterone Receptor (ER/PR) status was carried out in malignant lesions. AGNOR values could sharply distinguish benign from malignant lesions. Among the malignant lesions, an attempt to determine the value of AGNOR count in prognostication was made. AGNOR counts correlated with tumor size, mitoses and desmoplasia. ER/PgR negative tumors showed a tendency for high NOR counts, but lymph node metastasis, which is considered one of the most reliable prognostic indicators, did not concur with AGNOR counts in our study. These results indicate that AGNOR counts can not be used as a sole independent marker in breast cancer prognostication.
Subject(s)
Breast Diseases/pathology , Breast Neoplasms/ultrastructure , Case-Control Studies , Female , Humans , Nucleolus Organizer Region/pathology , Prognosis , Silver StainingABSTRACT
One hundred cervical tissues including 72 malignancies (68 squamous cell carcinomas, 3 adenocarcinomas, 1 neuro-endocrine carcinoma), 24 cases of CIN of various grades and 4 normals were examined for the presence of Human Papilloma virus (HPV) types by non isotopic in-situ hybridisation. Biotinylated probes to HPV types 16 and 18 were used in all the cases and 31 and 33 in 15 squamous Carcinomas. HPV DNA sequences were detected in 55/72 (76.4%) of the malignant lesions, of which among squamous Carcinomas. HPV 16 alone was present in 12 of 68 cases (17.64%) and type 18 in 15 of 68 cases (22.0%). 20/68 (29.4%) showed both types 16 and 18. Of the 15 cases examined for types 31 and 33.5 cases showed presence of both types. All three adenocarcinomas were negative for HPV 16, but positive for HPV 18. The solitary case of Neuroendocrine Carcinoma showed only HPV 18. Of the 24 CINS, type 16 was detected in 7/24 cases (29.1%) type 18 in 2/24 (8.3%) cases and both types in 1/24 (4.1%). None of the normal cases showed positive signals for HPV. The overall results show a slight preponderance of HPV 18 in this group of carcinoma of cervix studied and correlate with poor differentiation and greater aggressive behaviour of cervical cancer which is the most common type of cancer among women in this country.
Subject(s)
Uterine Cervical Dysplasia/pathology , DNA, Neoplasm/analysis , Female , Humans , In Situ Hybridization , India , Papillomaviridae/genetics , Uterine Cervical Neoplasms/pathologyABSTRACT
An unusual case of squamous carcinoma arising in the background of longstanding localised tracheolaryngeal amyloidosis is reported and the relevant literature is briefly reviewed.
Subject(s)
Amyloidosis/complications , Carcinoma, Squamous Cell/etiology , Humans , Laryngeal Diseases/complications , Laryngeal Neoplasms/etiology , Male , Middle Aged , Tracheal Diseases/complicationsABSTRACT
Benign breast tumours with a mixed cartilaginous and epithelial component are distinctly rare as evident from the literature. A case of Mixed Tumour of the breast presenting pre-operatively as a hard mass in a 65 year old male is reported. Histologically, it was composed of a mixture of benign cartilage, myoepithelial cells, tubules and a myxoid stroma in fat. A brief review of cartilage bearing lesions and mixed tumour in the mammary region is discussed.
Subject(s)
Adenoma, Pleomorphic/pathology , Aged , Breast Neoplasms/pathology , Humans , MaleABSTRACT
An extrarenal Wilms' tumour exhibiting an unusual histology is reported in the inguinal region of a 3 year old girl. The tumour presents a tubulopapillary pattern associated with foci of nephroblastomatosis, a morphologic variant not previously described in any of the extrarenal Wilms' tumour. The unusual histologic picture enhances the difficulty in histogenetic based precise diagnosis. This case represents the 8th EWT of the inguinal region.
Subject(s)
Child, Preschool , Female , Groin , Humans , Wilms Tumor/pathologyABSTRACT
Using indirect immuno-peroxidase staining technique, localization of immunoreactive follicle-stimulating hormone (IR-FSH) is demonstrated in the cytoplasm of the epithelial cells of normal human stomach. In view of their triangular shape and central nucleus and their predominance in the intermediate glands of the gastric mucosa, these cells are identified as parietal cells. The stromal tissue is devoid of staining reaction.